Benchtop Fractionisation Method for Subcellular Metabolomics


New fractionation technique for assigning metabolites to their subcellular localization developed by L. Fürtauer, W. Weckwerth and T. Nägele.

Although compartmentation is a key feature of eukaryotic cells, biological research is frequently limited by methods allowing for the comprehensive subcellular resolution of the metabolome. It has been widely accepted that such a resolution would be necessary in order to approximate cellular biochemistry and metabolic regulation, yet technical challenges still limit both the reproducible subcellular fractionation and the sample throughput being necessary for a statistically robust analysis. We present a method and a detailed protocol which is based on the non-aqueous fractionation technique enabling the assignment of metabolites to their subcellular localization.

⇒ Full Article on Frontiers

⇒ Editorial in "Laborjournal online"