Metabolomics: Methods and Protocols
Metabolomics: Methods and Protocols
Editor:
Weckwerth, Wolfram
Pages: 312
ISBN: 978-1588295613
Publisher:Humana Press
Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.
Part I of this volume focuses on GC-MS techniques; specifically, attention is given to
- metabolite profiling in blood plasma,
- construction and application of mass spectral and retention time index libraries,
- metabolomic profiling of natural volatiles.
In Part II, emphasis shifts to
- data integration and data mining techniques
- specific protocols discuss improving pattern recognition and biomarker selection for systems level approaches
- visualization and analysis of molecular and metabolomic data.
Parts III, IV, and V examine
- the coupling of capillary electrophoresis and mass spectrometry
- classical reversed phase liquid chromatography-mass spectrometry
- electrochemical detection of metabolites using HPLC separations coupled with coulometric electrode array detectors
In Part VI, protocols for the determination of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis is provided. The volume concludes with an examination of cutting-edge NMR protocols.
Table of contents:
- Metabolite Profiling in Blood Plasma
Oliver Fiehn, Tobias Kind
pp. 3-18 - Nonsupervised Construction and Application of Mass Spectral and Retention Time Index Libraries From Time-of-Flight Gas Chromatography–Mass Spectrometry Metabolite Profiles
Alexander Erban, Nicolas Schauer, Alisdair Fernie, Joachim Kopka
pp. 19-38 - Metabolomic Profiling of Natural Volatiles: Headspace Trapping: GC–MS
Yury M. Tikunov; Francel W. A. Verstappen; Robert D. Hall
pp. 39-54 - Integrative Profiling of Metabolites and Proteins: Improving Pattern Recognition and Biomarker Selection for Systems Level Approaches
Katja Morgenthal; Stefanie Wienkoop; Florian Wolschin; Wolfram Weckwerth
pp. 57-76 - Integrating Profiling Data: Using Linear Correlation to Reveal Coregulation of Transcript and Metabolites
Ewa Urbanczyk-Wochniak; Lothar Willmitzer; Alisdair R. Fernie
pp. 77-86 - Visualization and Analysis of Molecular Data
Matthias Scholz; Joachim Selbig
pp. 87-104 - A Gentle Guide to the Analysis of Metabolomic Data
Ralf Steuer; Katja Morgenthal; Wolfram Weckwerth; Joachim Selbig
pp. 105-126 - Capillary Electrophoresis–Mass Spectrometry for Metabolomics
Tomoyoshi Soga
pp. 129-138 - Application of Liquid Chromatography–Mass Spectrometry Analysis in Metabolomics: Reversed-Phase Monolithic Capillary Chromatography and Hydrophilic Chromatography Coupled to Electrospray Ionization–Mass Spectrometry
Vladimir V. Tolstikov; Oliver Fiehn; Nobuo Tanaka
pp. 141-156 - High-Performance Liquid Chromatography Separations Coupled With Coulometric Electrode Array Detectors: A Unique Approach to Metabolomics
Bruce S. Kristal; Yevgeniya I. Shurubor; Rima Kaddurah-Daouk; Wayne R. Matson
pp. 159-174 - Determination of Metabolic Flux Ratios From 13C-Experiments and Gas Chromatograpyh–Mass Spectrometry Data: Protocol and Principles
Annik Nanchen; Tobias Fuhrer; Uwe Sauer
pp. 177-198 - Understanding the Roadmap of Metabolism by Pathway Analysis
Stefan Schuster; Axel von Kamp; Mikhail Pachkov
pp. 199-226 - Revealing the Metabolome of Animal Tissues Using 1H Nuclear Magnetic Resonance Spectroscopy
Mark R. Viant
pp. 229-246 - Nuclear Magnetic Resonance Metabonomics: Methods for Drug Discovery and Development
Karl-Heinz Ott; Nelly Aranibar
pp. 247-272 - Practical Aspects of Uniform Stable Isotope Labeling of Higher Plants for Heteronuclear NMR-Based Metabolomics
Jun Kikuchi, Takashi Hirayama
pp. 273-286