The membrane proteome of male gametophyte in Solanum lycopersicum

Author(s)
Puneet Paul, Palak Chaturvedi, Mario Selymesi, Arindam Ghatak, Anida Mesihovic, Klaus-Dieter Scharf, Wolfram Weckwerth, Stefan Simm, Enrico Schleiff
Abstract

Pollen cells possess specialized cellular compartments separated by membranes. Consequently, mature pollen contains proteinaceous factors for inter- and intracellular transport of metabolites or ions to facilitate the upcoming energy exhausting processes-germination and fertilization. Despite the current advancement in the understanding of pollen development little is known about the role and molecular nature of the membrane proteome that participates in functioning and development of male gametophyte. We dissected the membrane proteome of mature pollen from economically important crop Solanum lycopersicum (tomato). Isolated membrane fractions from mature pollen of two tomato cultivars (cv. Moneymaker and cv. Red setter) were subjected to shotgun proteomics (GEL-LC-Orbitrap-MS). The global tomato protein assignment was achieved by mapping the peptides on reference genome (cv. Heinz 1706) and de nova assembled transcriptome based on mRNA sequencing from the respective cultivar. We identified 687 proteins, where 176 were assigned as putative membrane proteins. About 58% of the identified membrane proteins participate in transport processes. In depth analysis revealed proteins corresponding to energy related pathways (Glycolysis and Krebs cycle) as prerequisite for mature pollen, thereby revealing a reliable model of energy reservoir of the male gametophyte.

Biological significance: Mature pollen plays an indispensable role in plant fertility and crop production. To decipher the functionality of pollen global proteomics studies have been undertaken. However, these datasets are deficient in membrane proteins due to their low abundance and solubility. The work presented here provides a comprehensive investigation of membrane proteome of male gametophyte of an agriculturally important crop plant tomato. The analysis of membrane enriched fractions from two tomato cultivars ensured an effective profiling of the pollen membrane proteome. Particularly proteins of the Krebs cycle or the glycolysis process have been detected and thus a model for the energy dynamics and preparedness of the male gametophyte for the upcoming events-germination and fertilization is provided. (C) 2015 Elsevier B.V. All rights reserved.

Organisation(s)
Large-Instrument Facility for Mass Spectrometry in Life Sciences, Research Platform Vienna Metabolomics Center
External organisation(s)
Johann Wolfgang Goethe-Universität Frankfurt am Main, SPOT ITN Consortium, D.Y. Patil University
Journal
Journal of Proteomics
Volume
131
Pages
48-60
No. of pages
13
ISSN
1874-3919
DOI
https://doi.org/10.1016/j.jprot.2015.10.009
Publication date
01-2016
Peer reviewed
Yes
Austrian Fields of Science 2012
106037 Proteomics
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/the-membrane-proteome-of-male-gametophyte-in-solanum-lycopersicum(1c48af2b-ec3e-47b8-a8a1-55c1d18522ac).html