Vacuolar sucrose cleavage prevents limitation of cytosolic carbohydrate metabolism and stabilizes photosynthesis under abiotic stress

Author(s)
Jakob Weiszmann, Lisa Fürtauer, Wolfram Weckwerth, Thomas Nägele
Abstract

Stabilization of central carbohydrate metabolism plays a key role in plant stress response. Carbohydrates are substrate for numerous metabolic and stress-responsive reactions and have been shown to be involved in diverse signalling processes on a whole-plant level. Regulation of enzymatic sucrose synthesis and degradation is well-known to be central to many stress-related processes as it significantly impacts stress tolerance. Leaf sucrose metabolism involves sucrose cleavage by invertases and ATP-consuming resynthesis catalysed by hexokinase and sucrose phosphate synthase. These reactions establish a metabolic cycle. To study the physiological role of sucrose cycling, a kinetic model was developed to simulate dynamics of subcellular sugar concentrations in Arabidopsis thaliana under combined cold and high-light stress. Model simulation revealed that subcellular reprogramming of invertase-driven sucrose cleavage varies substantially between natural accessions of Arabidopsis which differ in their cold tolerance levels. A stress-induced shift of sucrose cleavage from the cytosol into the vacuole could only be observed for the tolerant accession while the susceptible accession increased the cytosolic proportion of sucrose cleavage. Under stress, reduction in vacuolar invertase activity significantly affected maximum quantum yield of photosystem II and CO2 assimilation rates. While wild-type plants circumvented a limitation of sucrose cleavage by increasing vacuolar invertase activity, mutant plants were not able to compensate their deficiency of vacuolar by cytosolic activity. Consequently, the capacity for cytosolic hexose generation was lower than for enzymatic hexose phosphorylation suggesting a role of vacuolar invertase activity in preventing a limitation in cytosolic hexose metabolism under stress. Enzymes: Invertase, EC 3.2.1.26; Hexokinase, EC 2.7.1.1.

Organisation(s)
Department of Ecogenomics and Systems Biology, Research Platform Vienna Metabolomics Center, Large-Instrument Facility for Mass Spectrometry in Life Sciences
Journal
FEBS Journal
Volume
285
Pages
4082-4098
No. of pages
17
ISSN
1742-464X
DOI
https://doi.org/10.1111/febs.14656
Publication date
11-2018
Publication status
Published
Peer reviewed
Yes
Austrian Fields of Science 2012
106031 Plant physiology, 106057 Metabolomics, 106002 Biochemistry
Keywords
Arabidopsis thaliana, energy metabolism, invertase, kinetic modelling, natural variation
ASJC Scopus subject areas
Biochemistry, Molecular Biology, Cell Biology
Portal url
https://ucris.univie.ac.at/portal/en/publications/vacuolar-sucrose-cleavage-prevents-limitation-of-cytosolic-carbohydrate-metabolism-and-stabilizes-photosynthesis-under-abiotic-stress(2371f24e-8af9-46d4-8644-4455acc9b974).html