Mass Western for absolute quantification of target proteins and considerations about the instrument of choice.
- Author(s)
- David Lyon, Wolfram Weckwerth, Stefanie Wienkoop
- Abstract
The Mass Western describes the absolute quantification of proteins based on stable isotope labeled integral standard peptides and liquid chromatography coupled selective reaction monitoring triple quadrupole mass spectrometry (LC-SRM/MS). Here, we present a detailed workflow including tips and we discuss advantages and disadvantages of using different types of MS for absolute quantification.
- Organisation(s)
- Volume
- 1072
- Pages
- 199-208
- No. of pages
- 10
- DOI
- https://doi.org/10.1007/978-1-62703-631-3_15
- Publication date
- 2014
- Austrian Fields of Science 2012
- 106037 Proteomics, 106023 Molecular biology, 106005 Bioinformatics, 106014 Genomics
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/mass-western-for-absolute-quantification-of-target-proteins-and-considerations-about-the-instrument-of-choice(3b02323f-fb6d-4922-b601-593e3c9546e6).html