Myo-inositol oxygenase is important for the removal of excess myo-inositol from syncytia induced by Heterodera schachtii in Arabidopsis roots
- Author(s)
- Shahid Siddique, Stefanie Endres, Miroslaw Sobczak, Zoran S. Radakovic, Lena Fragner, Florian M.W. Grundler, Wolfram Weckwerth, Raimund Tenhaken, Holger Bohlmann
- Abstract
The enzyme myo-inositol oxygenase is the key enzyme of a pathway leading from myo-inositol to UDP-glucuronic acid. In Arabidopsis, myo-inositol oxygenase is encoded by four genes. All genes are strongly expressed in syncytia induced by the beet cyst nematode Heterodera schachtii in Arabidopsis roots. Here, we studied the effect of a quadruple myo-inositol oxygenase mutant on nematode development. We performed metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant. The role of galactinol in syncytia was studied using Arabidopsis lines with elevated galactinol levels and by supplying galactinol to wild-type seedlings. The quadruple myo-inositol oxygenase mutant showed a significant reduction in susceptibility to H. schachtii, and syncytia had elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility could also be achieved by exogenous application of galactinol to wild-type seedlings. The primary function of myo-inositol oxygenase for syncytium development is probably not the production of UDP-glucuronic acid as a precursor for cell wall polysaccharides, but the reduction of myo-inositol levels and thereby a reduction in the galactinol level to avoid the induction of defence-related genes.
- Organisation(s)
- External organisation(s)
- Paris-Lodron Universität Salzburg, Warsaw University of Life Sciences (SGGW), Rheinische Friedrich-Wilhelms-Universität Bonn, Universität für Bodenkultur Wien
- Journal
- New Phytologist
- Volume
- 201
- Pages
- 476-485
- No. of pages
- 10
- ISSN
- 0028-646X
- DOI
- https://doi.org/10.1111/nph.12535
- Publication date
- 01-2014
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106002 Biochemistry, 106047 Animal ecology, 106030 Plant ecology
- Keywords
- ASJC Scopus subject areas
- Physiology, Plant Science
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/myoinositol-oxygenase-is-important-for-the-removal-of-excess-myoinositol-from-syncytia-induced-by-heterodera-schachtii-in-arabidopsis-roots(8922162e-9c35-465d-82dd-379facbf8cef).html