Integration of multi-omics data and deep phenotyping provides insights into responses to single and combined abiotic stress in potato
- Author(s)
- Maja Zagorščak, Lamis Abdelhakim, Natalia Yaneth Rodriguez-Granados, Jitka Široká, Arindam Ghatak, Carissa Bleker, Andrej Blejec, Jan Zrimec, Ondřej Novák, Aleš Pěnčík, Špela Baebler, Lucia Perez Borroto, Christian Schuy, Anže Županič, Leila Afjehi-Sadat, Bernhard Wurzinger, Wolfram Weckwerth, Maruša Pompe Novak, Marc R Knight, Miroslav Strnad, Christian Bachem, Palak Chaturvedi, Sophia Sonnewald, Rashmi Sasidharan, Klára Panzarová, Kristina Gruden, Markus Teige
- Abstract
Potato (Solanum tuberosum) is highly water and space efficient but susceptible to abiotic stresses such as heat, drought, and flooding, which are severely exacerbated by climate change. Our understanding of crop acclimation to abiotic stress, however, remains limited. Here, we present a comprehensive molecular and physiological high-throughput profiling of potato (Solanum tuberosum, cv. Désirée) under heat, drought, and waterlogging applied as single stresses or in combinations designed to mimic realistic future scenarios. Stress responses were monitored via daily phenotyping and multi-omics analyses of leaf samples comprising proteomics, targeted transcriptomics, metabolomics, and hormonomics at several timepoints during and after stress treatments. Additionally, critical metabolites of tuber samples were analyzed at the end of the stress period. We performed integrative multi-omics data analysis using a bioinformatic pipeline that we established based on machine learning and knowledge networks. Waterlogging produced the most immediate and dramatic effects on potato plants, interestingly activating ABA responses similar to drought stress. In addition, we observed distinct stress signatures at multiple molecular levels in response to heat or drought and to a combination of both. In response to all treatments, we found a downregulation of photosynthesis at different molecular levels, an accumulation of minor amino acids, and diverse stress-induced hormones. Our integrative multi-omics analysis provides global insights into plant stress responses, facilitating improved breeding strategies toward climate-adapted potato varieties.
- Organisation(s)
- Functional and Evolutionary Ecology, Core Facility Shared Services UBB
- External organisation(s)
- National Institute of Biology, Photon Systems Instruments, Utrecht University, Palacký University Olomouc, Wageningen University and Research Centre, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), University of Nova Gorica, Durham University
- Journal
- Plant Physiology
- Volume
- 197
- ISSN
- 0032-0889
- DOI
- https://doi.org/10.1093/plphys/kiaf126
- Publication date
- 04-2025
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106031 Plant physiology, 106044 Systems biology
- Keywords
- ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous), Physiology
- Sustainable Development Goals
- SDG 13 - Climate Action
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/4fb0252f-dcff-42a4-9186-957ba14b5f25