Haplotype-resolved genome assembly of the tetraploid potato cultivar Désirée
- Author(s)
- Tim Godec, Sebastian Beier, Natalia Yaneth Rodriguez-Granados, Rashmi Sasidharan, Lamis Abdelhakim, Markus Teige, Björn Usadel, Kristina Gruden, Marko Petek
- Abstract
Cultivar Désirée is an important model for potato functional genomics studies to assist breeding strategies. Here, we present a haplotype-resolved genome assembly of Désirée, achieved by assembling PacBio HiFi reads and Hi-C scaffolding, resulting in a high-contiguity chromosome-level assembly. We implemented a comprehensive annotation pipeline incorporating gene models and functional annotations from the Solanum tuberosum Phureja DM reference genome alongside RNA-seq reads to provide high-quality gene and transcript annotations. Additionally, we provide a genome-wide DNA methylation profile using Oxford Nanopore reads, enabling insights into potato epigenetics. The assembled genome, annotations, methylation and expression data are visualised in a publicly accessible genome browser, providing a valuable resource for the potato research community.
- Organisation(s)
- Department of Biochemistry and Cell Biology, Functional and Evolutionary Ecology
- External organisation(s)
- Jožef Stefan International Postgraduate School, Forschungszentrum Jülich, Utrecht University, Photon Systems Instruments, Heinrich-Heine-Universität Düsseldorf, National Institute of Biology
- Journal
- Scientific Data
- Volume
- 12
- Pages
- 1044
- ISSN
- 2052-4463
- DOI
- https://doi.org/10.1038/s41597-025-05372-3
- Publication date
- 06-2025
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106014 Genomics
- Keywords
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/be4c8d4b-da62-44e8-8492-83b6907795b5