Studying AMPK in an Evolutionary Context
- Author(s)
- Arpit Jain, Valentin Roustan, Wolfram Weckwerth, Ingo Ebersberger
- Abstract
The AMPK protein kinase forms the heart of a complex network controlling the metabolic activities in a eukaryotic cell. Unraveling the steps by which this pathway evolved from its primordial roots in the last eukaryotic common ancestor to its present status in contemporary species has the potential to shed light on the evolution of eukaryotes. A homolog search for the proteins interacting in this pathway is considerably straightforward. However, interpreting the results, when reconstructing the evolutionary history of the pathway over larger evolutionary distances, bears a number of pitfalls. With this in mind, we present a protocol to trace a metabolic pathway across contemporary species and backward in evolutionary time. Alongside the individual analysis steps, we provide guidelines for data interpretation generalizing beyond the analysis of AMPK.
- Organisation(s)
- Research Platform Vienna Metabolomics Center, Department of Biochemistry and Cell Biology
- External organisation(s)
- Johann Wolfgang Goethe-Universität Frankfurt am Main, Senckenberg Forschungsinstitut und Naturmuseum
- Journal
- Methods in Molecular Biology
- Volume
- 1732
- Pages
- 111-142
- No. of pages
- 32
- ISSN
- 1064-3745
- DOI
- https://doi.org/10.1007/978-1-4939-7598-3_8
- Publication date
- 2018
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106002 Biochemistry, 106031 Plant physiology, 106033 Phylogeny
- Keywords
- ASJC Scopus subject areas
- Genetics, Molecular Biology
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/studying-ampk-in-an-evolutionary-context(51a14bc1-a984-484d-b7a9-0907e30aae56).html