Induction and quantitative proteomic analysis of cell dedifferentiation during callus formation of lotus (Nelumbo nucifera Gaertn.spp. baijianlian)

Author(s)
Yanli Liu, Palak Chaturvedi, Jinlei Fu, Qingqing Cai, Wolfram Weckwerth, Pingfang Yang
Abstract

Lotus is an aquatic plant with high nutritional, ornamental and medical values. Its callus formation is crucial for germplasm innovation by genetic transformation. In this study, embryogenic callus was successfully induced on appropriate medium using cotyledons at 12 days after pollination as explants. To dissect cellular dedifferentiation and callus formation processes at the proteome level, cotyledons before and tissues from 10 to 20 days after induction were sampled for shotgun proteomic analysis. By applying multivariate statistics 91 proteins were detected as differentially regulated, and sorted into 6 functional groups according to MapMan ontology analysis. Most of these proteins were implicated in various metabolisms, demonstrating that plant cells underwent metabolism reprogramming during callus induction. 143% proteins were associated with stress and redox, indicating that the detached explants were subjected to a variety of stresses; 13.2% were cell and cell wall-related proteins, suggesting that these proteins played important roles in rapid cell division and proliferation. Some proteins were further evaluated at the mRNA levels by quantitative reverse transcription PCR analysis. In conclusion, the results contributed to further deciphering of molecular processes of cellular dedifferentiation and callus formation, and provided a reference data set for the establishment of transgenic transformation in lotus. (C) 2015 Elsevier B.V. All rights reserved.

Organisation(s)
Large-Instrument Facility for Mass Spectrometry in Life Sciences, Research Platform Vienna Metabolomics Center
External organisation(s)
Chinese Academy of Sciences (CAS), Huazhong Agricultural University
Journal
Journal of Proteomics
Volume
131
Pages
61-70
No. of pages
10
ISSN
1874-3919
DOI
https://doi.org/10.1016/j.jprot.2015.10.010
Publication date
01-2016
Peer reviewed
Yes
Austrian Fields of Science 2012
106037 Proteomics
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/induction-and-quantitative-proteomic-analysis-of-cell-dedifferentiation-during-callus-formation-of-lotus-nelumbo-nucifera-gaertnspp-baijianlian(01e0811c-663d-42b4-ab9d-22720c796393).html