Comprehensive tissue-specific proteome analysis of drought stress responses in Pennisetum glaucum (L.) R. Br. (Pearl millet)

Author(s)
Arindam Ghatak, Palak Chaturvedi, Matthias Nagler, Valentin Roustan, David Lyon, Gert Bachmann, Wolfgang Postl, Andreas Schroefl, Neetin Desai, Rajeev K. Varshney, Wolfram Weckwerth
Abstract

Pearl millet is the fifth most important cereal crop worldwide and cultivated especially by small holder farmers in arid and semi-arid regions because of its drought and salt tolerance. The molecular mechanisms of drought stress tolerance in Pennisetum remain elusive. We have used a shotgun proteomics approach to investigate protein signatures from different tissues under drought and control conditions. Drought stressed plants showed significant changes in stomatal conductance and increased root growth compared to the control plants. Root, leaf and seed tissues were harvested and 2281 proteins were identified and quantified in total. Leaf tissue showed the largest number of significant changes (120), followed by roots (25) and seeds (10). Increased levels of root proteins involved in cell wall-, lipid-, secondary- and signaling metabolism and the concomitantly observed increased root length point to an impaired shoot root communication under drought stress. The harvest index (HI) showed a significant reduction under drought stress. Proteins with a high correlation to the HI were identified using sparse partial least square (sPLS) analysis. Considering the importance of Pearl millet as a stress tolerant food crop, this study provides a first reference data set for future investigations of the underlying molecular mechanisms. Biological significance: Drought stress is the most limiting factor for plant growth and crop production worldwide. At the same time drought susceptible cereal crops are among the largest producers worldwide. In contrast, Pearl millet is a drought and salt tolerant cereal crop especially used in arid and semi-arid regions by small farmers. The multifactorial molecular mechanisms of this unique drought tolerance are not known. Here, we employ shotgun proteomics for a first characterization of the Pearl millet drought stress proteome. The experimental setup and the data set generated from this study reveal comprehensive physiological and proteomic responses of the drought stressed Pearl millet plants. Our study reveals statistically significant tissue-specific protein signatures during the adaptation to drought conditions. Thus, the work provides a first reference study of the drought stress proteome and related drought responsive proteins (DRP's) in Pearl millet. (C) 2016 Published by Elsevier B.V.

Organisation(s)
Large-Instrument Facility for Mass Spectrometry in Life Sciences, Research Platform Vienna Metabolomics Center
External organisation(s)
D.Y. Patil University, Amity University, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) India
Journal
Journal of Proteomics
Volume
143
Pages
122-135
No. of pages
14
ISSN
1874-3919
DOI
https://doi.org/10.1016/j.jprot.2016.02.032
Publication date
06-2016
Peer reviewed
Yes
Austrian Fields of Science 2012
106030 Plant ecology, 106037 Proteomics
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/comprehensive-tissuespecific-proteome-analysis-of-drought-stress-responses-in-pennisetum-glaucum-l-r-br-pearl-millet(0c03378b-fb62-4611-90b6-effb4c22dc95).html