Differential priming of Pisum sativum cultivars by AMF and Rhizobia

Reinhard Turetschek, Getinet Desalegn, Hans-Peter Kaul, Stefanie Wienkoop

Because of their nutritional value to man and life stock, legumes account for a big part of agricultural production. Due to their symbiotic interactions (Rhizobia & arbuscular mycorrhizal fungi - AMF) which enhance nutritional uptake, they substantially contribute to sustainable agriculture. Each legume is capable of forming symbiosis with particular Rhizobia and commonly several species of AMF. The interaction with Rhizobia is to a great extent controlled by the plant and each species shows different nodule morphology. With regard to breeding strategies, agronomy is interested in the effect of below ground parts on above ground traits (e.g. biomass, pathogen resistance levels, and yield). We tested the effect of single and co-inoculation with Rhizobia and AMF on the plants´ morphology as well as the leaf proteome and metabolome in two cultivars of P. sativum. The nodulation profile (weight and number of nodules) is remarkably distinct among cultivars and the proteome shows predominantly cultivar related effects rather than effects of the symbionts. However, we found that single Rhizobia or AMF inoculation showed the utmost effect on the proteome in a cultivar specific manner. As the intensity of the host-symbiont interaction over a plants´ lifespan usually varies between cultivars, we further aim to elucidate the nodules´ morphology as well as its proteome in a time series. These insights about cultivar specific symbiotic interaction provide knowledge for advanced sustainable breeding strategies.

Department of Ecogenomics and Systems Biology
External organisation(s)
Universität für Bodenkultur Wien
Publication date
Peer reviewed
Austrian Fields of Science 2012
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