Molecular response of Deinococcus radiodurans to simulated microgravity explored by proteometabolomic approach

Author(s)
Emanuel Ott, Felix M. Fuchs, Ralf Moeller, Ruth Hemmersbach, Yuko Kawaguchi, Akihiko Yamagishi, Wolfram Weckwerth, Tetyana Milojevic
Abstract

Regarding future space exploration missions and long-term exposure experiments, a detailed investigation of all factors present in the outer space environment and their effects on organisms of all life kingdoms is advantageous. Influenced by the multiple factors of outer space, the extremophilic bacterium Deinococcus radiodurans has been long-termly exposed outside the International Space Station in frames of the Tanpopo orbital mission. The study presented here aims to elucidate molecular key components in D. radiodurans, which are responsible for recognition and adaptation to simulated microgravity. D. radiodurans cultures were grown for two days on plates in a fast-rotating 2-D clinostat to minimize sedimentation, thus simulating reduced gravity conditions. Subsequently, metabolites and proteins were extracted and measured with mass spectrometry-based techniques. Our results emphasize the importance of certain signal transducer proteins, which showed higher abundances in cells grown under reduced gravity. These proteins activate a cellular signal cascade, which leads to differences in gene expressions. Proteins involved in stress response, repair mechanisms and proteins connected to the extracellular milieu and the cell envelope showed an increased abundance under simulated microgravity. Focusing on the expression of these proteins might present a strategy of cells to adapt to microgravity conditions.

Organisation(s)
Department of Biophysical Chemistry, Large-Instrument Facility for Mass Spectrometry in Life Sciences, Department of Ecogenomics and Systems Biology
External organisation(s)
Deutsches Zentrum für Luft- und Raumfahrt e.V. (DLR), Chiba Inst Technol, Chiba Institute of Technology, PERC, Tokyo Institute of Technology (TIT)
Journal
Scientific Reports
Volume
9
No. of pages
13
ISSN
2045-2322
DOI
https://doi.org/10.1038/s41598-019-54742-6
Publication date
12-2019
Peer reviewed
Yes
Austrian Fields of Science 2012
Biochemistry, Proteomics
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/molecular-response-of-deinococcus-radiodurans-to-simulated-microgravity-explored-by-proteometabolomic-approach(0fdc2ef4-3d20-43c6-9c93-9d159bd7c528).html