Diurnal effects of anoxia on the metabolome of the seagrass Zostera marina

Author(s)
Harald Hasler-Sheetal, Lena Fragner, Marianne Holmer, Wolfram Weckwerth
Abstract

We investigated the response, adaptation and tolerance mechanisms of the temperate seagrass Zostera marina to water column anoxia. We exposed Z. marina to a diurnal light/dark cycle under anoxia and assessed the metabolic response by measuring the metabolome with gas chromatography coupled to mass spectrometry (GC–MS). During anoxia and light exposure the roots showed an altered metabolome whereas the leaves were only marginally affected, indicating that photosynthetically derived oxygen could satisfy the oxygen demand in the leaves but not in the roots. Nocturnal anoxia caused a biphasic shift in the metabolome of roots and leaves. The first phase, after 15 h under anoxia and 3 h of darkness showed a fast increase of lactate, pyruvate, GABA (γ-aminobutyric acid), succinate, alanine and a decrease in glutamate and glutamine. The second phase, after 21 h under anoxia and 9 h of darkness showed a decrease in lactate and pyruvate and an increase in alanine, GABA and succinate. This reprogramming of the metabolome after 21 h under anoxia indicates a possible mitigation mechanism to avoid the toxic effects of anoxia. A pathway enrichment analysis proposes the alanine shunt, the GABA shunt and the 2-oxoglutarate shunt as such mitigation mechanisms that alleviate pyruvate levels and lead to carbon and nitrogen storage during anoxia. This work demonstrates the applicability of metabolomics to assess low oxygen stress responses of Z. marina and allows us to propose an anoxia recovery model.

Organisation(s)
Large-Instrument Facility for Mass Spectrometry in Life Sciences
External organisation(s)
University of Southern Denmark (SDU)
Journal
Metabolomics
Volume
11
Pages
1208-1218
No. of pages
11
ISSN
1573-3882
DOI
https://doi.org/10.1007/s11306-015-0776-9
Publication date
10-2015
Peer reviewed
Yes
Austrian Fields of Science 2012
106002 Biochemistry, 106031 Plant physiology, 106008 Botany
Keywords
ASJC Scopus subject areas
Biochemistry, Clinical Biochemistry, Endocrinology, Diabetes and Metabolism
Portal url
https://ucris.univie.ac.at/portal/en/publications/diurnal-effects-of-anoxia-on-the-metabolome-of-the-seagrass-zostera-marina(1b7f6d13-9c04-4c20-80be-9611c9d28986).html