Metabolomic and Proteomic Profiles Reveal the Dynamics of Primary Metabolism during Seed Development of Lotus (Nelumbo nucifera)

Author(s)
Lei Wang, Jinlei Fu, Ming Li, Lena Fragner, Wolfram Weckwerth, Pingfang Yang
Abstract

Sacred lotus (Nelumbo nucifera) belongs to the Nelumbonaceae family. Its seeds are widely consumed in Asian countries as snacks or even medicine. Besides the market value, lotus seed also plays a crucial role in the lotus life cycle. Consequently, it is essential to gain a comprehensive understanding of the development of lotus seed. During its development, lotus seed undergoes cell division, expansion, reserve accumulation, desiccation, and maturation phases. We observed morphological and biochemical changes from 10 to 25 days after pollination (DAP) which corresponded to the reserve synthesis and accumulation phase. The volume of the seed expanded until 20 DAP with the color of the seed coat changing from yellow-green to dark green and gradually fading again. Starch and protein rapidly accumulated from 15 to 20 DAP. To further reveal metabolic adaptation, primary metabolites and proteins profiles were obtained using mass spectrometry based platforms. Metabolites and enzymes involved in sugar metabolism, glycolysis, TCA cycle and amino acid metabolism showed sequential dynamics enabling the clear separation of the different metabolic states during lotus seed development. The integration of the data revealed a highly significant metabolic switch at 15 DAP going through a transition of metabolically highly active tissue to the preparation of storage tissue. The results provide a reference data set for the evaluation of primary metabolism during lotus seed development.

Organisation(s)
Large-Instrument Facility for Mass Spectrometry in Life Sciences, Research Platform Vienna Metabolomics Center
External organisation(s)
Chinese Academy of Sciences (CAS)
Journal
Frontiers in Plant Science
Volume
7
No. of pages
12
ISSN
1664-462X
DOI
https://doi.org/10.3389/fpls.2016.00750
Publication date
06-2016
Peer reviewed
Yes
Austrian Fields of Science 2012
106002 Biochemistry
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/metabolomic-and-proteomic-profiles-reveal-the-dynamics-of-primary-metabolism-during-seed-development-of-lotus-nelumbo-nucifera(321ec085-510d-4051-a0da-75cfb8626af2).html