Exploring natural variation of Pinus pinaster Aiton using metabolomics

Author(s)
Monica Meijon, Isabel Feito, Michal Oravec, Carolina Delatorre, Wolfram Weckwerth, Juan Majada, Luis Valledor
Abstract

Natural variation of the metabolome of Pinus pinaster was studied to improve understanding of its role in the adaptation process and phenotypic diversity. The metabolomes of needles and the apical and basal section of buds were analysed in ten provenances of P.pinaster, selected from France, Spain and Morocco, grown in a common garden for 5years. The employment of complementary mass spectrometry techniques (GC-MS and LC-Orbitrap-MS) together with bioinformatics tools allowed the reliable quantification of 2403 molecular masses. The analysis of the metabolome showed that differences were maintained across provenances and that the metabolites characteristic of each organ are mainly related to amino acid metabolism, while provenances were distinguishable essentially through secondary metabolism when organs were analysed independently. Integrative analyses of metabolome, environmental and growth data provided a comprehensive picture of adaptation plasticity in conifers. These analyses defined two major groups of plants, distinguished by secondary metabolism: that is, either Atlantic or Mediterranean provenance. Needles were the most sensitive organ, where strong correlations were found between flavonoids and the water regime of the geographic origin of the provenance. The data obtained point to genome specialization aimed at maximizing the drought stress resistance of trees depending on their origin.

Organisation(s)
Large-Instrument Facility for Mass Spectrometry in Life Sciences, Research Platform Vienna Metabolomics Center
External organisation(s)
Reg Inst Res & Agrofood Dev Asturias, Expt Stn La Mata, Czech Academy of Sciences, Forest & Wood Technol Res Ctr, Expt Stn La Mata, Universidad de Oviedo
Journal
Molecular Ecology
Volume
25
Pages
959-976
No. of pages
18
ISSN
0962-1083
DOI
https://doi.org/10.1111/mec.13525
Publication date
02-2016
Peer reviewed
Yes
Austrian Fields of Science 2012
106014 Genomics
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/exploring-natural-variation-of-pinus-pinaster-aiton-using-metabolomics(585d446c-fed3-48e5-a159-247d01dd98c2).html