Boron nitride as desalting material in combination with phosphopeptide enrichment in shotgun proteomics
- Author(s)
- Takeshi Furuhashi, Ella Nukarinen, Shigenori Ota, Wolfram Weckwerth
- Abstract
Hydrophilic peptides in shotgun proteomics have been shown to be problematic in conventional chromatography. Typically, C18 solid phase extraction or peptide traps are used for desalting the sample prior to mass spectrometry analysis, but the capacity to retain hydrophilic peptides is not very high, causing a bias toward more hydrophobic peptides. This is particularly problematic in phosphoproteomic studies. We tested the compatibility of commercially available boron nitride as a novel material for peptide desalting. Boron nitride can be used to recover a wide range of peptides with different physicochemical properties comparable to combined C18 and graphite carbon material.
- Organisation(s)
- External organisation(s)
- GL Sciences Inc.
- Journal
- Analytical Biochemistry
- Volume
- 452
- Pages
- 16-18
- No. of pages
- 3
- ISSN
- 0003-2697
- DOI
- https://doi.org/10.1016/j.ab.2014.01.005
- Publication date
- 05-2014
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106002 Biochemistry, 106037 Proteomics, 104002 Analytical chemistry
- Keywords
- ASJC Scopus subject areas
- Molecular Biology, Biophysics, Biochemistry, Cell Biology
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/boron-nitride-as-desalting-material-in-combination-with-phosphopeptide-enrichment-in-shotgun-proteomics(856eca1e-29e4-474b-9a6b-ac2bcfcf04a1).html