Tissue-Specific Proteome and Subcellular Microscopic Analyses Reveal the Effect of High Salt Concentration on Actin Cytoskeleton and Vacuolization in Aleurone Cells during Early Germination of Barley
- Author(s)
- Georgi-Atanasov Dermendjiev, Madeleine Schnurer, Jakob Weiszmann, Sarah Wilfinger, Emanuel Ott, Claudia Gebert, Wolfram Weckwerth, Verena Ibl
- Abstract
Cereal grain germination provides the basis for crop production and requires a tissue-specific interplay between the embryo and endosperm during heterotrophic germination involving signalling, protein secretion, and nutrient uptake until autotrophic growth is possible. High salt concentrations in soil are one of the most severe constraints limiting the germination of crop plants, affecting the metabolism and redox status within the tissues of germinating seed. However, little is known about the effect of salt on seed storage protein mobilization, the endomembrane system, and protein trafficking within and between these tissues. Here, we used mass spectrometry analyses to investigate the protein dynamics of the embryo and endosperm of barley (Hordeum vulgare, L.) at five different early points during germination (0, 12, 24, 48, and 72 h after imbibition) in germinated grains subjected to salt stress. The expression of proteins in the embryo as well as in the endosperm was temporally regulated. Seed storage proteins (SSPs), peptidases, and starch-digesting enzymes were affected by salt. Additionally, microscopic analyses revealed an altered assembly of actin bundles and morphology of protein storage vacuoles (PSVs) in the aleurone layer. Our results suggest that besides the salt-induced protein expression, intracellular trafficking and actin cytoskeleton assembly are responsible for germination delay under salt stress conditions.
- Organisation(s)
- Functional and Evolutionary Ecology, Research Platform Vienna Metabolomics Center, Department of Microbiology, Immunobiology and Genetics
- External organisation(s)
- Universität Wien
- Journal
- International Journal of Molecular Sciences
- Volume
- 22
- No. of pages
- 20
- ISSN
- 1422-0067
- DOI
- https://doi.org/10.3390/ijms22179642
- Publication date
- 09-2021
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106008 Botany, 106023 Molecular biology, 106037 Proteomics, 106031 Plant physiology
- Keywords
- ASJC Scopus subject areas
- Molecular Biology, Spectroscopy, Catalysis, Inorganic Chemistry, Computer Science Applications, Physical and Theoretical Chemistry, Organic Chemistry
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/tissuespecific-proteome-and-subcellular-microscopic-analyses-reveal-the-effect-of-high-salt-concentration-on-actin-cytoskeleton-and-vacuolization-in-aleurone-cells-during-early-germination-of-barley(9b6adcfa-c312-409b-b3ea-840a4d8af07f).html