Xylan Localization on Pulp and Viscose Fiber Surfaces

Author(s)
Christian Hutterer, Karin Fackler, Gabriele Schild, Verena Ibl, Antje Potthast
Abstract

A method for the immunological visualization of plant polysaccharides in native plant tissues was adopted for the histological investigation of xylan on kraft pulp and xylan-enriched viscose fibers. The method consisted of the selective labeling of xylan structures through the binding of specific monoclonal primary antibodies and fluorescein isothiocyanate (FITC)carrying secondary antibodies. This indirect immunolabeling method was adapted for pulp and viscose fibers through the blockage of unspecific binding sites with bovine serum albumin (BSA), which allowed a selective localization of xylan. The combination of this technique with high resolution confocal laser scanning microscopy (CLSM) rendered a parallel detection of morphological changes of pulp fibers alongside various processing steps possible. Within this study, kraft pulps from birch, beech, and eucalyptus were investigated throughout a purification process that enabled an upgrade from paper pulps to dissolving pulps by caustic hemicellulose extraction and xylan-enriched viscose fibers. The method demonstrated its potential for gaining novel insights into pulp purification, as well as fiber modification through the application of an immunolabeling method.

Organisation(s)
Department of Ecogenomics and Systems Biology
External organisation(s)
Kompetenzzentrum Holz GmbH, Lenzing AG, Universität für Bodenkultur Wien
Journal
BioResources
Volume
12
Pages
5632-5648
No. of pages
17
ISSN
1930-2126
DOI
https://doi.org/10.15376/biores.12.3.5632-5648
Publication date
2017
Peer reviewed
Yes
Austrian Fields of Science 2012
Plant anatomy, Phytochemistry, Ultrastructure research
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/xylan-localization-on-pulp-and-viscose-fiber-surfaces(c162e21e-508a-464f-9501-a60626d404ae).html