The Potato Yam Phyllosphere Ectosymbiont Paraburkholderia sp. Msb3 Is a Potent Growth Promotor in Tomato

Author(s)
Johannes B. Herpell, Florian Schindler, Mersad Bejtović, Lena Fragner, Bocar Diallo, Anke Bellaire, Susanne Kublik, Bärbel U. Foesel, Silvia Gschwendtner, Melina Kerou, Michael Schloter, Wolfram Weckwerth
Abstract

The genus Paraburkholderia includes a variety of species with promising features for sustainable biotechnological solutions in agriculture through increasing crop productivity. Here, we present a novel Paraburkholderia isolate, a permanent and predominant member of the Dioscoreae bulbifera (yam family, Dioscoreaceae) phyllosphere, making up to 25% of the microbial community on leaf acumens. The 8.5 Mbp genome of isolate Msb3 encodes an unprecedented combination of features mediating a beneficial plant-associated lifestyle, including biological nitrogen fixation (BNF), plant hormone regulation, detoxification of various xenobiotics, degradation of aromatic compounds and multiple protein secretion systems including both T3SS and T6SS. The isolate exhibits significant growth promotion when applied to agriculturally important plants such as tomato, by increasing the total dry biomass by up to 40%. The open question about the “beneficial” nature of this strain led us to investigate ecological and generic boundaries in Burkholderia sensu lato. In a refined phylogeny including 279 Burkholderia sensu lato isolates strain Msb3 clusters within Clade I Paraburkholderia, which also includes few opportunistic strains that can potentially act as pathogens, as revealed by our ecological meta-data analysis. In fact, we demonstrate that all genera originating from the “plant beneficial and environmental” (PBE) Burkholderia species cluster include opportunists. This indicates that further functional examinations are needed before safe application of these strains in sustainable agricultural settings can be assured.

Organisation(s)
Functional and Evolutionary Ecology, Large-Instrument Facility for Mass Spectrometry in Life Sciences, Research Platform Vienna Metabolomics Center
External organisation(s)
Universität Wien
Journal
Frontiers in Microbiology
Volume
581
Pages
581
No. of pages
1
ISSN
1664-302X
DOI
https://doi.org/10.3389/fmicb.2020.00581
Publication date
04-2020
Peer reviewed
Yes
Austrian Fields of Science 2012
106022 Microbiology, 405001 Agroecology, 106044 Systems biology
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/the-potato-yam-phyllosphere-ectosymbiont-paraburkholderia-sp-msb3-is-a-potent-growth-promotor-in-tomato(c230382e-9d8a-4e76-92c4-6c3bc0b71571).html