An evolutionary view on thylakoid protein phosphorylation uncovers novel phosphorylation hotspots with potential functional implications

Author(s)
Michele Grieco, Arpit Jain, Ingo Ebersberger, Markus Teige
Abstract

The regulation of photosynthetic light reactions by reversible protein phosphorylation is well established today, but functional studies have so far mostly been restricted to processes affecting light-harvesting complex II and the core proteins of photosystem II. Virtually no functional data are available on regulatory effects at the other photosynthetic complexes despite the identification of multiple phosphorylation sites. Therefore we summarize the available data from 50 published phospho-proteomics studies covering the main complexes involved in photosynthetic light reactions in the 'green lineage' (i.e. green algae and land plants) as well as its cyanobacterial counterparts. In addition, we performed an extensive orthologue search for the major photosynthetic thylakoid proteins in 41 sequenced genomes and generated sequence alignments to survey the phylogenetic distribution of phosphorylation sites and their evolutionary conservation from green algae to higher plants. We observed a number of uncharacterized phosphorylation hotspots at photosystem I and the ATP synthase with potential functional relevance as well as an unexpected divergence of phosphosites. Although technical limitations might account for a number of those differences, we think that many of these phosphosites have important functions. This is particularly important for mono- and dicot plants, where these sites might be involved in regulatory processes such as stress acclimation.

Organisation(s)
Department of Biochemistry and Cell Biology
External organisation(s)
Johann Wolfgang Goethe-Universität Frankfurt am Main, Senckenberg Forschungsinstitut und Naturmuseum
Journal
Journal of Experimental Botany
Volume
67
Pages
3883-96
No. of pages
14
ISSN
0022-0957
DOI
https://doi.org/10.1093/jxb/erw164
Publication date
06-2016
Peer reviewed
Yes
Austrian Fields of Science 2012
106008 Botany, 106031 Plant physiology
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/an-evolutionary-view-on-thylakoid-protein-phosphorylation-uncovers-novel-phosphorylation-hotspots-with-potential-functional-implications(c34ea2ce-8554-4ea0-ab88-3648f1ad5266).html