Subcellular dynamics of proteins and metabolites under abiotic stress reveal deferred response of the Arabidopsis thaliana hexokinase-1 mutant gin2-1 to high light

Author(s)
Lisa Kuestner, Lisa Fuertauer, Wolfram Weckwerth, Thomas Naegele, Arnd G. Heyer
Abstract

Stress responses in plants imply spatio-temporal changes in enzymes and metabolites, including subcellular compartment-specific re-allocation processes triggered by sudden changes in environmental parameters. To investigate interactions of primary metabolism with abiotic stress, the gin2-1 mutant, defective in the sugar sensor hexokinase 1 (HXK1) was compared with its wildtype Landsberg erecta (Ler) based on time resolved, compartment-specific metabolome and proteome data obtained over a full diurnal cycle. The high light sensitive gin2-1 mutant was substantially delayed in subcellular re-distribution of metabolites upon stress, and this correlated with a massive reduction in proteins belonging to the ATP producing electron transport chain under high light, while fewer changes occurred in the cold. In the wildtype, compounds specifically protecting individual compartments could be identified, e.g., maltose and raffinose in plastids, myo-inositol in mitochondria, but gin2-1 failed to recruit these substances to the respective compartments, or responded only slowly to high irradiance. No such delay was obtained in the cold. At the whole cell level, concentrations of the amino acids, glycine and serine, provided strong evidence for an important role of the photorespiratory pathway during stress exposure, and different subcellular allocation of serine may contribute to the slow growth of the gin2-1 mutant under high irradiance.

Organisation(s)
Functional and Evolutionary Ecology, Large-Instrument Facility for Mass Spectrometry in Life Sciences, Department of Ecogenomics and Systems Biology
External organisation(s)
Universität Stuttgart, Ludwig-Maximilians-Universität München
Journal
The Plant Journal
Volume
100
Pages
456-472
No. of pages
17
ISSN
0960-7412
DOI
https://doi.org/10.1111/tpj.14491
Publication date
08-2019
Peer reviewed
Yes
Austrian Fields of Science 2012
Proteomics, Metabolomics , Plant physiology
Keywords
ASJC Scopus subject areas
,
Portal url
https://ucris.univie.ac.at/portal/en/publications/subcellular-dynamics-of-proteins-and-metabolites-under-abiotic-stress-reveal-deferred-response-of-the-arabidopsis-thaliana-hexokinase1-mutant-gin21-to-high-light(cb85e1b9-dec5-487d-ad10-fe5cb25e5a8d).html