Mass Spectrometry Untangles Plant Membrane Protein Signaling Networks

Author(s)
Yanmei Chen, Wolfram Weckwerth
Abstract

Plasma membranes (PMs) act as primary cellular checkpoints for sensing signals and controlling solute transport. Membrane proteins communicate with intracellular processes through protein interaction networks. Deciphering these signaling networks provides crucial information for elucidating in vivo cellular regulation. Large-scale proteomics enables system-wide characterization of the membrane proteome, identification of ligand-receptor pairs, and elucidation of signals originating at membranes. In this review we assess recent progress in the development of mass spectrometry (MS)-based proteomic pipelines for determining membrane signaling pathways. We focus in particular on current techniques for the analysis of membrane protein phosphorylation and interaction, and how these proteins may be connected to downstream changes in gene expression, metabolism, and physiology.

Organisation(s)
Research Platform Vienna Metabolomics Center, Large-Instrument Facility for Mass Spectrometry in Life Sciences, Functional and Evolutionary Ecology
External organisation(s)
China Agricultural University, Universität Wien
Journal
Trends in Plant Science
Volume
25
Pages
930-944
No. of pages
15
ISSN
1360-1385
DOI
https://doi.org/10.1016/j.tplants.2020.03.013
Publication date
09-2020
Peer reviewed
Yes
Austrian Fields of Science 2012
106002 Biochemistry
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/mass-spectrometry-untangles-plant-membrane-protein-signaling-networks(dfa8bd4b-578c-483e-a506-6d0b07484df0).html