Glycolate induces redox tuning of photosystem II in vivo

Author(s)
Marine Messant, Stefan Timm, Andrea Fantuzzi, Wolfram Weckwerth, Hermann Bauwe, A. William Rutherford, Anja Krieger-Liszkay
Abstract

Bicarbonate removal from the nonheme iron at the acceptor side of photosystem II (PSII) was shown recently to shift the midpoint potential of the primary quinone acceptor QA to a more positive potential and lowers the yield of singlet oxygen (1O2) production. The presence of QA

results in weaker binding of bicarbonate, suggesting a redox-based regulatory and protective mechanism where loss of bicarbonate or exchange of bicarbonate by other small carboxylic acids may protect PSII against1O2 in vivo under photorespiratory conditions. Here, we compared the properties of QA in the Arabidopsis (Arabidopsis thaliana) photorespiration mutant deficient in peroxisomal HYDROXYPYRUVATE REDUCTASE1 (hpr1-1), which accumulates glycolate in leaves, with the wild type. Photosynthetic electron transport was affected in the mutant, and chlorophyll fluorescence showed slower electron transport between QA and QB in the mutant. Glycolate induced an increase in the temperature maximum of thermoluminescence emission, indicating a shift of the midpoint potential of QA to a more positive value. The yield of1O2 production was lowered in thylakoid membranes isolated from hpr1-1 compared with the wild type, consistent with a higher potential of QA/QA

. In addition, electron donation to photosystem I was affected in hpr1-1 at higher light intensities, consistent with diminished electron transfer out of PSII. This study indicates that replacement of bicarbonate at the nonheme iron by a small carboxylate anion occurs in plants in vivo. These findings suggested that replacement of the bicarbonate on the nonheme iron by glycolate may represent a regulatory mechanism that protects PSII against photooxidative stress under low-CO2 conditions.

Organisation(s)
Department of Ecogenomics and Systems Biology, Research Platform Vienna Metabolomics Center, Large-Instrument Facility for Mass Spectrometry in Life Sciences
Journal
Plant Physiology
Volume
177
Pages
1277-1285
No. of pages
9
ISSN
0032-0889
DOI
https://doi.org/10.1104/pp.18.00341
Publication date
06-2018
Publication status
Published
Peer reviewed
Yes
Austrian Fields of Science 2012
106002 Biochemistry, 106044 Systems biology, 106023 Molecular biology, 106032 Photobiology
ASJC Scopus subject areas
Physiology, Genetics, Plant Science
Portal url
https://ucris.univie.ac.at/portal/en/publications/glycolate-induces-redox-tuning-of-photosystem-ii-in-vivo(e12107bd-df92-45de-bc34-27f79bc1fc71).html