Didymella pinodes impact on shoot and nodule development in Pisum sativum

Reinhard Turetschek, Stefanie Wienkoop, Hans-Peter Kaul, Getinet Desalegn, Tamara Epple

Field pea is one of the major protein sources and due to its association with root-symbionts especially meaningful for crop rotation and winter greening. Biotic stress, such as above ground infestation by the necrotrophic fungi Didymella pinodes reduces yields globally. On the plants level, the impact of infection reaches down to the interaction with the symbionts. The development of nodules is affected because of the different needs of above ground parts. However, cultivars exhibit a variety of nodule development, which is, among other things, based on the shoots morphology as well as on the onset of blooming. Here, we examined the nodule development of two field pea cultivars with different resistance levels to D. pinodes at 24 h, 48 h and 2 weeks post infection by counting and weighing nodules with subsequent extraction of primary Metabolites (GC-MS) and label free quantification of proteins (Orbitrap Elite). The different nodule development is consistent with the distinguishable shoot morphology and the onset of blooming of each cultivar. Additionally, the formation of shoot nodes post infection appears to depend on the microbial association of the roots. Moreover, we found that the weight of nodules after infection is maintained when co-inoculation with arbuscular mycorrhizal fungi is given. Thus, we presume that nodule development post infection is interrelated with mycorrhizal linkage of the roots. Whether cultivar related nodule development after pathogenic attack shows an impact on yield remains to be elucidated.

External organisation(s)
Universität für Bodenkultur Wien
Publication date
Peer reviewed
Austrian Fields of Science 2012
401112 Plant protection, 106037 Proteomics, 106034 Phytochemistry
ASJC Scopus subject areas
Agricultural and Biological Sciences(all)
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