Coordination Complex Formation and Redox Properties of Kynurenic and Xanthurenic Acid Can Affect Brain Tissue Homeodynamics

Author(s)
Gert Bachmann, Vladimir Chobot, Lenka Kubicova, Wolfram Weckwerth, Franz Hadacek
Abstract

Reactive oxygen species (ROS) are known for their participation in various physiological and pathological processes in organisms, including ageing or degeneration. Kynurenine pathway metabolites, such as kynurenic (KYNA) or xanthurenic (XA) acid, can affect neurodegenerative diseases due to their ROS scavenging and Fe ion coordination complex formation but insights are still incomplete. Therefore, we investigated the formation and antioxidant capabilities of KYNA- and XA-Fe complexes by nano-electrospray-mass spectrometry, differential pulse voltammetry, deoxyribose degradation and Fe-II autoxidation assays. XA formed coordination complexes with Fe-II or Fe-III ions and was an effective antioxidant. By contrast, only Fe-II-KYNA complexes could be detected. Moreover, KYNA showed no antioxidant effects in the FeCl3/ascorbic acid deoxyribose degradation assay variant and only negligible activities in the Fe-II autoxidation assay. Coordination complexes of Fe ions with KYNA probably stabilize KYNA in its keto tautomer form. Nevertheless, both KYNA and XA exhibited sufficient antioxidant activities in some of the employed assay variants. The results provide evidence that both have the potential to alleviate neurodegenerative diseases by helping to maintain tissue redox homeodynamics.

Organisation(s)
Functional and Evolutionary Ecology, Department of Microbiology and Ecosystem Science
Journal
Antioxidants
Volume
8
No. of pages
13
ISSN
2076-3921
DOI
https://doi.org/10.3390/antiox8100476
Publication date
10-2019
Peer reviewed
Yes
Austrian Fields of Science 2012
104005 Electrochemistry, 106002 Biochemistry
Keywords
ASJC Scopus subject areas
Molecular Biology, Biochemistry, Physiology, Clinical Biochemistry, Cell Biology
Portal url
https://ucris.univie.ac.at/portal/en/publications/coordination-complex-formation-and-redox-properties-of-kynurenic-and-xanthurenic-acid-can-affect-brain-tissue-homeodynamics(f9a09ca9-a961-4795-9dbb-faec37b4c47c).html