Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s

Author(s)
Valentin Roustan, Julia Hilscher, Marieluise Weidinger, Siegfried Reipert, Azita Shabrangy, Claudia Gebert, Bianca Dietrich, Georgi Dermendjiev, Madeleine Schnurer, Pierre-Jean Roustan, Eva Stoger, Verena Ibl
Abstract

Cereal endosperm is a short-lived tissue adapted for nutrient storage, containing specialized organelles, such as protein bodies (PBs) and protein storage vacuoles (PSVs), for the accumulation of storage proteins. During development, protein trafficking and storage require an extensive reorganization of the endomembrane system. Consequently, endomembrane-modifying proteins will influence the final grain quality and yield. However, little is known about the molecular mechanism underlying endomembrane system remodeling during barley grain development. By using label-free quantitative proteomics profiling, we quantified 1,822 proteins across developing barley grains. Based on proteome annotation and a homology search, 94 proteins associated with the endomembrane system were identified that exhibited significant changes in abundance during grain development. Clustering analysis allowed characterization of three different development phases; notably, integration of proteomics data with in situ subcellular microscopic analyses showed a high abundance of cytoskeleton proteins associated with acidified PBs at the early development stages. Moreover, endosomal sorting complex required for transport (ESCRT)-related proteins and their transcripts are most abundant at early and mid-development. Specifically, multivesicular bodies (MVBs), and the ESCRT-III HvSNF7 proteins are associated with PBs during barley endosperm development. Together our data identified promising targets to be genetically engineered to modulate seed storage protein accumulation that have a growing role in health and nutritional issues.

Organisation(s)
Core Facility of Cell Imaging and Ultrastructure Research, Department of Ecogenomics and Systems Biology, Functional and Evolutionary Ecology, Department of Microbiology, Immunobiology and Genetics
External organisation(s)
Universität für Bodenkultur Wien, Medizinische Universität Wien
Journal
Scientific Reports
Volume
10
ISSN
2045-2322
DOI
https://doi.org/10.1038/s41598-020-58740-x
Publication date
02-2020
Peer reviewed
Yes
Austrian Fields of Science 2012
Cell biology
Portal url
https://ucris.univie.ac.at/portal/en/publications/protein-sorting-into-protein-bodies-during-barley-endosperm-development-is-putatively-regulated-by-cytoskeleton-members-mvbs-and-the-hvsnf7s(fd505ba7-a5bd-4366-a7f6-88c63bc5470c).html