Combining microdilution with MicroResp (TM): Microbial substrate utilization, antimicrobial susceptibility and respiration
- Author(s)
- Sigrid Drage, Doris Engelmeier, Gert Bachmann, Angela Sessitsch, Birgit Mitter, Franz Hadacek
- Abstract
Pharmacological studies focus on susceptibility of pathogenic microbes against specific drugs or combinations of them, ecological studies on substrate utilization efficiency of variable microbial communities. The Micro-Resp (TM) system was especially developed to study soil microbial communities. It was slightly modified to facilitate exploring of microbial growth efficiency in a concentration-dependent fashion (microdilutions of carbohydrate mixtures or specific toxic chemicals). After turbidimetric growth assessment, calorimetric indicator plates (cresol red agar) were mounted to the assay plates. The substrate utilisation design is illustrated by glucose and a plant carbohydrate mixture, the antimicrobial susceptibility design by the naphthoquinone juglone. Dose-response effects are explored by curve fitting of nonlinear models that especially have been developed to detect hormetic effects that are characterized by stimulation at lower followed by inhibition at higher dosages (U- and inverse U-shaped effects). Multivariate analyses are presented utilizing metavariables that were obtained in the curve fitting process of the measured parameters growth and respiration and the factor growth efficiency.
- Organisation(s)
- External organisation(s)
- Austrian Institute of Technology
- Journal
- Journal of Microbiological Methods
- Volume
- 88
- Pages
- 399-412
- No. of pages
- 14
- ISSN
- 0167-7012
- DOI
- https://doi.org/10.1016/j.mimet.2012.01.006
- Publication date
- 2012
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106022 Microbiology
- Sustainable Development Goals
- SDG 12 - Responsible Consumption and Production
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/8da9db5b-337c-4b78-8826-1773a9892f6d