Ubiquitin lysine 63 chain-forming ligases regulate apical dominance in Arabidopsis
- Author(s)
- Xiao-Jun Yin, Sara Volk, Karin Ljung, Norbert Mehlmer, Karel Dolezal, Franck Ditengou, Shigeru Hanano, Seth J. Davis, Elmon Schmelzer, Göran Sandberg, Markus Teige, Klaus Palme, Cecile Pickart, Andreas Bachmair
- Abstract
Lys-63-linked multiubiquitin chains play important roles in signal transduction in yeast and in mammals, but the functions for this type of chain in plants remain to be defined. The RING domain protein RGLG2 (for RING domain Ligase2) from Arabidopsis thaliana can be N-terminally myristoylated and localizes to the plasma membrane. It can form Lys-63-linked multiubiquitin chains in an in vitro reaction. RGLG2 has overlapping functions with its closest sequelog, RGLG1, and single mutants in either gene are inconspicuous. rglg1 rglg2 double mutant plants exhibit loss of apical dominance and altered phyllotaxy, two traits critically influenced by the plant hormone auxin. Auxin and cytokinin levels are changed, and the plants show a decreased response to exogenously added auxin. Changes in the abundance of PIN family auxin transport proteins and synthetic lethality with a mutation in the auxin transport regulator BIG suggest that the directional flow of auxin is modulated by RGLG activity. Modification of proteins by Lys-63-linked multiubiquitin chains is thus important for hormone-regulated, basic plant architecture. © 2007 American Society of Plant Biologists.
- Organisation(s)
- Department of Biochemistry and Cell Biology
- External organisation(s)
- Max-Planck-Institut für Pflanzenzüchtungsforschung, Johns Hopkins University, Swedish University of Agricultural Sciences (SLU), Palacký University Olomouc (UP), Umeå University, Université de Fribourg
- Journal
- The Plant Cell
- Volume
- 19
- Pages
- 1898-1911
- No. of pages
- 14
- ISSN
- 1040-4651
- DOI
- https://doi.org/10.1105/tpc.107.052035
- Publication date
- 2007
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106002 Biochemistry
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/ubiquitin-lysine-63-chainforming-ligases-regulate-apical-dominance-in-arabidopsis(14fb89f3-ff37-4640-8065-3d22a4c3398a).html