Procyanidin A1 and its digestive products inhibit acrylamide-induced IPEC-J2 cell damage: apoptosis and cell cycle arrest
- Author(s)
- Fangfang Yan, Si Quin, Chengmin Wang, Wolfram Weckwerth, Qun Lu, Rui Liu
- Abstract
Our previous study has demonstrated that procyanidin A1 (A1) and its simulated digestive product (D-A1) can prevent acrylamide (ACR)-induced cytotoxicity in small intestine cells. However, the potential mechanism remains poorly understood. In this study, ACR treatment was found to increase the levels of 8-hydroxy-deoxyguanine (8-OHdG) and phosphorated histone H2AX (γH2AX), two DNA damage markers, thereby resulting in cell cycle arrest in the G2/M phase; whereas both A1 and D-A1 could prevent the phosphorylation of ataxia telangiectasia mutated (ATM) and checkpoint kinase 2 (Chk2), and then regulate the expression of G2/M phase-related proteins, finally maintaining normal cell cycle progression. Moreover, A1 and D-A1 could increase the B cell lymphoma 2 (Bcl-2)/Bcl2-associated X (Bax) ratio and decrease the expression of cleaved caspase-3 and cleaved caspase-9 proteins to alleviate ACR-induced cell apoptosis, which might be related to the inhibition of the mitogen-activated protein kinase (MAPK) pathway. More importantly, A1 showed no remarkable variation in inhibitory effect before and after digestion, indicating that it can endure gastrointestinal digestion and may be a promising phytochemical to alleviate ACR-induced intestinal cell damage.
- Organisation(s)
- Functional and Evolutionary Ecology
- External organisation(s)
- Hunan Agricultural University, Huazhong Agricultural University
- Journal
- Food Science and Human Wellness
- ISSN
- 2213-4530
- DOI
- https://doi.org/10.26599/FSHW.2024.9250272
- Publication date
- 09-2024
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 301303 Medical biochemistry
- Keywords
- ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Sustainable Development Goals
- SDG 3 - Good Health and Well-being
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/3f2a74ac-6fe3-4745-93c9-9c4ea4f0be91