Protocol for Enrichment of the Membrane Proteome of Mature Tomato Pollen.
- Author(s)
- Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff
- Abstract
We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.
- Organisation(s)
- External organisation(s)
- Johann Wolfgang Goethe-Universität Frankfurt am Main
- Journal
- Bio-Protocol
- Volume
- 7
- No. of pages
- 9
- ISSN
- 2331-8325
- DOI
- https://doi.org/10.21769/BioProtoc.2315
- Publication date
- 2017
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106002 Biochemistry, 106037 Proteomics
- Keywords
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/5ec7a087-bed9-4654-bda3-e0ca3784e975