Protocol for Enrichment of the Membrane Proteome of Mature Tomato Pollen.

Author(s)
Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff
Abstract

We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.

Organisation(s)
Research Platform Vienna Metabolomics Center
External organisation(s)
Johann Wolfgang Goethe-Universität Frankfurt am Main
Journal
Bio-Protocol
Volume
7
No. of pages
9
ISSN
2331-8325
DOI
https://doi.org/10.21769/BioProtoc.2315
Publication date
2017
Peer reviewed
Yes
Austrian Fields of Science 2012
106002 Biochemistry, 106037 Proteomics
Keywords
Portal url
https://ucrisportal.univie.ac.at/en/publications/protocol-for-enrichment-of-the-membrane-proteome-of-mature-tomato-pollen(5ec7a087-bed9-4654-bda3-e0ca3784e975).html