Evidence for novel tomato seed allergens: Ige-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling
- Author(s)
- Olivia Y. Bässler, Julia Weiss, Stefanie Wienkoop, Karola Lehmann, Christian Scheler, Sabine Dölle, Dietmar Schwarz, Philipp Franken, Eckhard George, Margitta Worm, Wolfram Weckwerth
- Abstract
Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomatosensitized
patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin
(Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher
than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional
protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were
purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying
IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology
between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins
- Organisation(s)
- External organisation(s)
- Max-Planck-Institut für Molekulare Pflanzenphysiologie, Universität Potsdam, Proteome Factory AG, Charité – Universitätsmedizin Berlin, Leibniz-Institut für Gemüse- und Zierpflanzenbau Großbeeren/Erfurt e. V.
- Journal
- Journal of Proteome Research
- Volume
- 8
- Pages
- 1111-1122
- No. of pages
- 12
- ISSN
- 1535-3893
- DOI
- https://doi.org/10.1021/pr800186d
- Publication date
- 2009
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106002 Biochemistry, 1030 Physics, Astronomy, 106037 Proteomics
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/evidence-for-novel-tomato-seed-allergens-igereactive-legumin-and-vicilin-proteins-identified-by-multidimensional-protein-fractionationmass-spectrometry-and-in-silico-epitope-modeling(dc4289a3-3f37-4de0-9503-dcf06fac104a).html