Heat-induced proteomic changes in anthers of contrasting rice genotypes under variable stress regimes
- Author(s)
- Ritesh Kumar, Arindam Ghatak, Isha Goyal, Neelam K. Sarkar, Wolfram Weckwerth, Anil Grover, Palak Chaturvedi
- Abstract
Heat stress drastically affects anther tissues resulting in poor plant fertility, necessitating an urgent need to determine the key proteome regulation associated with mature anther in response to heat stress. We identified several genotype - specific protein alterations in rice anthers of Moroberekan (Japonica, heat sensitive), IR64 (Indica, moderately heat tolerant), and Nagina22 (Aus, heat tolerant) in the short-term (ST_HS; one cycle of 42°C, 4 hours before anthesis) and long-term (LT_HS; 6 cycles of 38°C, 6 hours before anthesis) heat stress. The proteins upregulated in long-term heat stress in Nagina22 were enriched in biological processes related to unfolded protein binding and carboxylic acid metabolism, including amino acid metabolism. In short-term heat stress, Nagina22 anthers were enriched in proteins associated with vitamin E biosynthesis and GTPase activator activity. In contrast, downregulated proteins were related to ribosomal proteins. The expression of different Hsp20 and DnaJ was genotype specific. Overall, the heat response in Nagina22 was associated with its capacity for adequate metabolic control and cellular homeostasis, which may be critical for its higher reproductive thermotolerance. This study improves our understanding of thermotolerance mechanisms in rice anthers during anthesis and lays a foundation for breeding thermotolerant varieties via molecular breeding.
- Organisation(s)
- Research Platform Vienna Metabolomics Center, Functional and Evolutionary Ecology
- External organisation(s)
- University of Delhi
- Journal
- Frontiers in Plant Science
- Volume
- 13
- ISSN
- 1664-462X
- DOI
- https://doi.org/10.3389/fpls.2022.1083971
- Publication date
- 01-2023
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106037 Proteomics, 106031 Plant physiology, 106044 Systems biology, 106014 Genomics
- Keywords
- ASJC Scopus subject areas
- Plant Science
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/heatinduced-proteomic-changes-in-anthers-of-contrasting-rice-genotypes-under-variable-stress-regimes(e4ff4627-d7ac-42c3-9224-d5b1cc4612d5).html