Recent advances and challenges in potato improvement using CRISPR/Cas genome editing
- Author(s)
- Izabela Anna Chincinska, Magdalena Miklaszewska, Dorota Sołtys-Kalina
- Abstract
Main conclusion: Genome editing using CRISPR/Cas technology improves the quality of potato as a food crop and enables its use as both a model plant in fundamental research and as a potential biofactory for producing valuable compounds for industrial applications. Abstract: Potato (Solanum tuberosum L.) plays a significant role in ensuring global food and nutritional security. Tuber yield is negatively affected by biotic and abiotic stresses, and enzymatic browning and cold-induced sweetening significantly contribute to post-harvest quality losses. With the dual challenges of a growing population and a changing climate, potato enhancement is essential for its sustainable production. However, due to several characteristics of potato, including high levels of heterozygosity, tetrasomic inheritance, inbreeding depression, and self-incompatibility of diploid potato, conventional breeding practices are insufficient to achieve substantial trait improvement in tetraploid potato cultivars within a relatively short time. CRISPR/Cas-mediated genome editing has opened new possibilities to develop novel potato varieties with high commercialization potential. In this review, we summarize recent developments in optimizing CRISPR/Cas-based methods for potato genome editing, focusing on approaches addressing the challenging biology of this species. We also discuss the feasibility of obtaining transgene-free genome-edited potato varieties and explore different strategies to improve potato stress resistance, nutritional value, starch composition, and storage and processing characteristics. Altogether, this review provides insight into recent advances, possible bottlenecks, and future research directions in potato genome editing using CRISPR/Cas technology.
- Organisation(s)
- Functional and Evolutionary Ecology
- External organisation(s)
- University of Gdańsk, Plant Breeding and Acclimatization Institute-National Research Institute
- Journal
- Planta
- Volume
- 257
- No. of pages
- 27
- ISSN
- 0032-0935
- DOI
- https://doi.org/10.1007/s00425-022-04054-3
- Publication date
- 12-2022
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106014 Genomics, 106013 Genetics, 401113 Plant breeding
- Keywords
- ASJC Scopus subject areas
- Genetics, Plant Science
- Sustainable Development Goals
- SDG 13 - Climate Action
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/recent-advances-and-challenges-in-potato-improvement-using-crisprcas-genome-editing(f4cc4984-3994-4775-9f33-a3b656f1d5e1).html